Detailed information of TA system TA134285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2199335..2200038
• Replicon: chromosome
• Accession: NZ_CP041871
• Organism: Mycobacterium tuberculosis strain 1-0021P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ35_RS10145
• Protein ID: WP_003409778.1
• Coordinates: 2199335..2199664 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ35_RS10150
• Protein ID: WP_003409780.1
• Coordinates: 2199661..2200038 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ35_RS10125	2195718..2196788	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPJ35_RS10130	2196785..2197300	+	516	WP_003409718.1	flavin reductase family protein	-
FPJ35_RS10135	2197297..2198358	+	1062	WP_193076333.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ35_RS10140	2198355..2199125	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ35_RS10145	2199335..2199664	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ35_RS10150	2199661..2200038	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ35_RS10155	2200035..2200625	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ35_RS10160	2200680..2202044	+	1365	WP_003903691.1	HNH endonuclease	-
FPJ35_RS10165	2202199..2202651	-	453	WP_003899095.1	lipoprotein	-
FPJ35_RS10170	2202715..2203116	+	402	WP_003409869.1	hypothetical protein	-
FPJ35_RS10175	2203109..2203291	-	183	WP_003409870.1	hypothetical protein	-
FPJ35_RS10180	2203405..2203755	-	351	WP_003409871.1	hypothetical protein	-
FPJ35_RS10185	2203766..2204668	-	903	WP_003899097.1	hypothetical protein	-
FPJ35_RS10190	2204689..2204880	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T134285 WP_003409778.1 NZ_CP041871:c2199664-2199335 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T134285 NZ_CP041871:c2199664-2199335 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT134285 WP_003409780.1 NZ_CP041871:c2200038-2199661 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT134285 NZ_CP041871:c2200038-2199661 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8