Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1951275..1951888 Replicon chromosome
Accession NZ_CP041871
Organism Mycobacterium tuberculosis strain 1-0021P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag FPJ35_RS09025 Protein ID WP_003408465.1
Coordinates 1951275..1951664 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag FPJ35_RS09030 Protein ID WP_003408469.1
Coordinates 1951661..1951888 (-) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ35_RS08995 1946904..1947818 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
FPJ35_RS09000 1947821..1948651 + 831 WP_003408448.1 cyclase family protein -
FPJ35_RS09005 1948651..1949001 + 351 WP_003898986.1 cupin domain-containing protein -
FPJ35_RS09010 1949054..1949872 + 819 WP_193076327.1 3-keto-5-aminohexanoate cleavage protein -
FPJ35_RS09015 1949886..1950665 + 780 WP_003408460.1 IclR family transcriptional regulator -
FPJ35_RS09025 1951275..1951664 - 390 WP_003408465.1 PIN domain-containing protein Toxin
FPJ35_RS09030 1951661..1951888 - 228 WP_003408469.1 antitoxin Antitoxin
FPJ35_RS09035 1952106..1953590 + 1485 WP_193076328.1 hypothetical protein -
FPJ35_RS09040 1953587..1954834 + 1248 WP_003408476.1 serine hydrolase -
FPJ35_RS09045 1954877..1955296 - 420 WP_003408483.1 hypothetical protein -
FPJ35_RS09050 1955286..1955996 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-116)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T134283 WP_003408465.1 NZ_CP041871:c1951664-1951275 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T134283 NZ_CP041871:c1951664-1951275 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT134283 WP_003408469.1 NZ_CP041871:c1951888-1951661 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT134283 NZ_CP041871:c1951888-1951661 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9


Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References