Detailed information of TA system TA134268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 755678..756311
• Replicon: chromosome
• Accession: NZ_CP041871
• Organism: Mycobacterium tuberculosis strain 1-0021P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ35_RS03460
• Protein ID: WP_015629650.1
• Coordinates: 755678..756061 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FPJ35_RS03465
• Protein ID: WP_003403368.1
• Coordinates: 756156..756311 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ35_RS03435	750970..751506	+	537	WP_003403341.1	50S ribosomal protein L10	-
FPJ35_RS03440	751543..751935	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FPJ35_RS03445	751928..752623	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FPJ35_RS03450	752694..754199	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ35_RS03455	754280..755290	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ35_RS03460	755678..756061	-	384	WP_015629650.1	hypothetical protein	Toxin
FPJ35_RS03465	756156..756311	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ35_RS03470	756387..757103	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ35_RS03475	757379..757687	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ35_RS03480	757674..757919	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FPJ35_RS03485	758029..758466	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FPJ35_RS03490	758463..758717	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ35_RS03495	758831..761194	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13886.68 Da
• Isoelectric Point: 6.0803

>T134268 WP_015629650.1 NZ_CP041871:c756061-755678 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRLIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T134268 NZ_CP041871:c756061-755678 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTTGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT134268 WP_003403368.1 NZ_CP041871:c756311-756156 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT134268 NZ_CP041871:c756311-756156 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8