Detailed information of TA system TA134216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242063..1242631
• Replicon: chromosome
• Accession: NZ_CP041870
• Organism: Mycobacterium tuberculosis strain 1-0023P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ36_RS05865
• Protein ID: WP_003405865.1
• Coordinates: 1242257..1242631 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ36_RS05860
• Protein ID: WP_003405863.1
• Coordinates: 1242063..1242260 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ36_RS05840	1238104..1238742	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ36_RS05845	1238832..1239839	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ36_RS05850	1239856..1240839	-	984	WP_003898731.1	hypothetical protein	-
FPJ36_RS05855	1240902..1241975	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ36_RS05860	1242063..1242260	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ36_RS05865	1242257..1242631	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ36_RS05870	1242834..1243532	+	699	WP_003898733.1	hypothetical protein	-
FPJ36_RS05875	1243650..1243835	+	186	WP_003901093.1	hypothetical protein	-
FPJ36_RS05880	1243762..1244037	-	276	WP_003405867.1	hypothetical protein	-
FPJ36_RS05885	1244280..1244603	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ36_RS05890	1244618..1245328	-	711	WP_003911399.1	hypothetical protein	-
FPJ36_RS05895	1245350..1245559	+	210	WP_003911400.1	hypothetical protein	-
FPJ36_RS05900	1245511..1246218	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T134216 WP_003405865.1 NZ_CP041870:1242257-1242631 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T134216 NZ_CP041870:1242257-1242631 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT134216 WP_003405863.1 NZ_CP041870:1242063-1242260 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT134216 NZ_CP041870:1242063-1242260 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07