Detailed information of TA system TA134121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2891938..2892575
• Replicon: chromosome
• Accession: NZ_CP041868
• Organism: Mycobacterium tuberculosis strain 1-0030P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ38_RS13420
• Protein ID: WP_003413180.1
• Coordinates: 2891938..2892333 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPJ38_RS13425
• Protein ID: WP_003413183.1
• Coordinates: 2892330..2892575 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ38_RS13380	2888023..2888682	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ38_RS13385	2888679..2889338	+	660	WP_003900847.1	hypothetical protein	-
FPJ38_RS13390	2889335..2889997	+	663	WP_003900848.1	hypothetical protein	-
FPJ38_RS13395	2889994..2890272	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ38_RS13400	2890365..2890778	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ38_RS13405	2890817..2891074	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ38_RS13410	2891071..2891448	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ38_RS13415	2891464..2891838	-	375	WP_003413177.1	hypothetical protein	-
FPJ38_RS13420	2891938..2892333	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ38_RS13425	2892330..2892575	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ38_RS13430	2892986..2893405	-	420	WP_003413190.1	A24 family peptidase	-
FPJ38_RS13435	2893417..2894226	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ38_RS13440	2894223..2895476	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ38_RS13445	2895469..2895981	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T134121 WP_003413180.1 NZ_CP041868:c2892333-2891938 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T134121 NZ_CP041868:c2892333-2891938 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT134121 WP_003413183.1 NZ_CP041868:c2892575-2892330 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT134121 NZ_CP041868:c2892575-2892330 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31