Detailed information of TA system TA134003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3067330..3068009
• Replicon: chromosome
• Accession: NZ_CP041866
• Organism: Mycobacterium tuberculosis strain 1-0036P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ40_RS14390
• Protein ID: WP_003414059.1
• Coordinates: 3067330..3067746 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ40_RS14395
• Protein ID: WP_003414061.1
• Coordinates: 3067743..3068009 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ40_RS14370	3063382..3064284	-	903	WP_031647842.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ40_RS14375	3064353..3065105	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ40_RS14380	3065349..3065624	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ40_RS14385	3065621..3067243	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ40_RS14390	3067330..3067746	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ40_RS14395	3067743..3068009	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ40_RS14400	3068035..3068430	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ40_RS14405	3068427..3068696	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ40_RS14410	3068706..3069800	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ40_RS14415	3069797..3070216	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ40_RS14420	3070290..3070769	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ40_RS14425	3070840..3071640	-	801	WP_003911953.1	thymidylate synthase	-
FPJ40_RS14430	3071796..3072533	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T134003 WP_003414059.1 NZ_CP041866:c3067746-3067330 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T134003 NZ_CP041866:c3067746-3067330 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT134003 WP_003414061.1 NZ_CP041866:c3068009-3067743 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT134003 NZ_CP041866:c3068009-3067743 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64