Detailed information of TA system TA134000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2868312..2868949
• Replicon: chromosome
• Accession: NZ_CP041866
• Organism: Mycobacterium tuberculosis strain 1-0036P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ40_RS13300
• Protein ID: WP_003413180.1
• Coordinates: 2868312..2868707 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPJ40_RS13305
• Protein ID: WP_003413183.1
• Coordinates: 2868704..2868949 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ40_RS13260	2864397..2865056	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ40_RS13265	2865053..2865712	+	660	WP_003900847.1	hypothetical protein	-
FPJ40_RS13270	2865709..2866371	+	663	WP_003900848.1	hypothetical protein	-
FPJ40_RS13275	2866368..2866646	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ40_RS13280	2866739..2867152	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ40_RS13285	2867191..2867448	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ40_RS13290	2867445..2867822	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ40_RS13295	2867838..2868212	-	375	WP_003413177.1	hypothetical protein	-
FPJ40_RS13300	2868312..2868707	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ40_RS13305	2868704..2868949	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ40_RS13310	2869360..2869779	-	420	WP_003413190.1	A24 family peptidase	-
FPJ40_RS13315	2869791..2870600	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ40_RS13320	2870597..2871850	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ40_RS13325	2871843..2872355	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T134000 WP_003413180.1 NZ_CP041866:c2868707-2868312 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T134000 NZ_CP041866:c2868707-2868312 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT134000 WP_003413183.1 NZ_CP041866:c2868949-2868704 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT134000 NZ_CP041866:c2868949-2868704 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31