Detailed information of TA system TA133969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 758518..759310
• Replicon: chromosome
• Accession: NZ_CP041866
• Organism: Mycobacterium tuberculosis strain 1-0036P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ40_RS03475
• Protein ID: WP_003403386.1
• Coordinates: 758873..759310 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ40_RS03470
• Protein ID: WP_003403381.1
• Coordinates: 758518..758763 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ40_RS03440	753537..755043	+	1507	Protein_679	carotenoid cleavage oxygenase	-
FPJ40_RS03445	755124..756134	+	1011	WP_031647733.1	ABC transporter ATP-binding protein	-
FPJ40_RS03450	756522..756905	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ40_RS03455	757000..757155	-	156	WP_031582830.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ40_RS03460	757231..757947	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ40_RS03465	758223..758531	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ40_RS03470	758518..758763	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ40_RS03475	758873..759310	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ40_RS03480	759307..759561	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ40_RS03485	759675..762038	+	2364	WP_031582834.1	arylsulfatase AtsD	-
FPJ40_RS03490	762101..762427	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ40_RS03495	762339..762677	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ40_RS03500	762674..762847	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T133969 WP_003403386.1 NZ_CP041866:c759310-758873 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T133969 NZ_CP041866:c759310-758873 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT133969 WP_003403381.1 NZ_CP041866:c758763-758518 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT133969 NZ_CP041866:c758763-758518 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0