Detailed information of TA system TA133961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 697843..698489
• Replicon: chromosome
• Accession: NZ_CP041866
• Organism: Mycobacterium tuberculosis strain 1-0036P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: FPJ40_RS03110
• Protein ID: WP_003403122.1
• Coordinates: 697843..698235 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPJ40_RS03115
• Protein ID: WP_031647732.1
• Coordinates: 698235..698489 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ40_RS03095	693505..695031	+	1527	WP_003403112.1	virulence factor Mce family protein	-
FPJ40_RS03100	695094..696236	+	1143	WP_003911253.1	virulence factor Mce family protein	-
FPJ40_RS03105	696241..697791	+	1551	WP_003403119.1	MCE family protein	-
FPJ40_RS03110	697843..698235	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
FPJ40_RS03115	698235..698489	-	255	WP_031647732.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
FPJ40_RS03120	698672..699907	-	1236	WP_003403128.1	ATP-binding protein	-
FPJ40_RS03125	700158..700571	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
FPJ40_RS03130	700568..700804	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ40_RS03135	700908..701414	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FPJ40_RS03140	701528..702058	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPJ40_RS03145	702042..702803	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FPJ40_RS03150	702860..703171	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14112.14 Da
• Isoelectric Point: 4.6558

>T133961 WP_003403122.1 NZ_CP041866:c698235-697843 [Mycobacterium tuberculosis]
MNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T133961 NZ_CP041866:c698235-697843 [Mycobacterium tuberculosis]
ATGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9494.85 Da
• Isoelectric Point: 7.0175

>AT133961 WP_031647732.1 NZ_CP041866:c698489-698235 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVR

Nucleotide

• Length: 255 bp

>AT133961 NZ_CP041866:c698489-698235 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4