Detailed information of TA system TA133918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239531..1240099
• Replicon: chromosome
• Accession: NZ_CP041865
• Organism: Mycobacterium tuberculosis strain 1-0039P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ41_RS05850
• Protein ID: WP_003405865.1
• Coordinates: 1239725..1240099 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ41_RS05845
• Protein ID: WP_003405863.1
• Coordinates: 1239531..1239728 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ41_RS05825	1235572..1236210	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ41_RS05830	1236300..1237307	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ41_RS05835	1237324..1238307	-	984	WP_003898731.1	hypothetical protein	-
FPJ41_RS05840	1238370..1239443	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ41_RS05845	1239531..1239728	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ41_RS05850	1239725..1240099	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ41_RS05855	1240302..1241000	+	699	WP_003898733.1	hypothetical protein	-
FPJ41_RS05860	1241118..1241303	+	186	WP_003901093.1	hypothetical protein	-
FPJ41_RS05865	1241230..1241505	-	276	WP_003405867.1	hypothetical protein	-
FPJ41_RS05870	1241748..1242071	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ41_RS05875	1242086..1242796	-	711	WP_003911399.1	hypothetical protein	-
FPJ41_RS05880	1242818..1243027	+	210	WP_003911400.1	hypothetical protein	-
FPJ41_RS05885	1242979..1243686	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T133918 WP_003405865.1 NZ_CP041865:1239725-1240099 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T133918 NZ_CP041865:1239725-1240099 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT133918 WP_003405863.1 NZ_CP041865:1239531-1239728 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT133918 NZ_CP041865:1239531-1239728 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07