Detailed information of TA system TA133912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 755227..756019
• Replicon: chromosome
• Accession: NZ_CP041865
• Organism: Mycobacterium tuberculosis strain 1-0039P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ41_RS03460
• Protein ID: WP_003403386.1
• Coordinates: 755582..756019 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ41_RS03455
• Protein ID: WP_003403381.1
• Coordinates: 755227..755472 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ41_RS03425	750247..751752	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ41_RS03430	751833..752843	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ41_RS03435	753231..753614	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ41_RS03440	753709..753864	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ41_RS03445	753940..754656	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ41_RS03450	754932..755240	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ41_RS03455	755227..755472	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ41_RS03460	755582..756019	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ41_RS03465	756016..756270	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ41_RS03470	756384..758747	+	2364	WP_003901895.1	arylsulfatase AtsD	-
FPJ41_RS03475	758810..759136	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ41_RS03480	759048..759386	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ41_RS03485	759383..759556	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T133912 WP_003403386.1 NZ_CP041865:c756019-755582 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T133912 NZ_CP041865:c756019-755582 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT133912 WP_003403381.1 NZ_CP041865:c755472-755227 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT133912 NZ_CP041865:c755472-755227 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0