Detailed information of TA system TA133888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3090417..3090987
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ42_RS14590
• Protein ID: WP_003414166.1
• Coordinates: 3090417..3090773 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ42_RS14595
• Protein ID: WP_003901465.1
• Coordinates: 3090757..3090987 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS14570	3085869..3087557	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FPJ42_RS14575	3087561..3087887	-	327	WP_003414157.1	hypothetical protein	-
FPJ42_RS14580	3088060..3088647	+	588	WP_003914429.1	DUF3558 family protein	-
FPJ42_RS14585	3088666..3090315	+	1650	WP_031654722.1	CocE/NonD family hydrolase	-
FPJ42_RS14590	3090417..3090773	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ42_RS14595	3090757..3090987	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ42_RS14600	3091030..3092073	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ42_RS14605	3092264..3092539	+	276	WP_193093033.1	DUF1778 domain-containing protein	-
FPJ42_RS14610	3092715..3092969	-	255	WP_003917684.1	hypothetical protein	-
FPJ42_RS14615	3093117..3093521	+	405	WP_009938577.1	hypothetical protein	-
FPJ42_RS14620	3093518..3093709	+	192	WP_003414184.1	hypothetical protein	-
FPJ42_RS14625	3093773..3095062	+	1290	Protein_2886	transposase family protein	-
FPJ42_RS14630	3095296..3095553	+	258	WP_003899489.1	hypothetical protein	-
FPJ42_RS14635	3095658..3095969	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3090417..3103824	13407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T133888 WP_003414166.1 NZ_CP041864:c3090773-3090417 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T133888 NZ_CP041864:c3090773-3090417 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT133888 WP_003901465.1 NZ_CP041864:c3090987-3090757 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT133888 NZ_CP041864:c3090987-3090757 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3