Detailed information of TA system TA133870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2187966..2188510
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TLU9
• Locus tag: FPJ42_RS10140
• Protein ID: WP_003409896.1
• Coordinates: 2187966..2188262 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P67299
• Locus tag: FPJ42_RS10145
• Protein ID: WP_003409899.1
• Coordinates: 2188259..2188510 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS10090	2183834..2184262	-	429	Protein_1993	hypothetical protein	-
FPJ42_RS10095	2184283..2184474	-	192	WP_003409876.1	hypothetical protein	-
FPJ42_RS10100	2184475..2184771	-	297	WP_003409877.1	hypothetical protein	-
FPJ42_RS10105	2185011..2185226	+	216	WP_003409878.1	antitoxin	-
FPJ42_RS10110	2185223..2185534	+	312	WP_003409881.1	type II toxin-antitoxin system VapC family toxin	-
FPJ42_RS10115	2185508..2186029	-	522	WP_003904745.1	hypothetical protein	-
FPJ42_RS10120	2186004..2186381	+	378	WP_010886136.1	type II toxin-antitoxin system toxin HigB	-
FPJ42_RS10125	2186423..2186872	+	450	WP_003409886.1	type II toxin-antitoxin system antitoxin HigA	-
FPJ42_RS10130	2186869..2187414	+	546	WP_003409891.1	SecB-like chaperone	-
FPJ42_RS10135	2187303..2187917	-	615	WP_003901296.1	hypothetical protein	-
FPJ42_RS10140	2187966..2188262	-	297	WP_003409896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ42_RS10145	2188259..2188510	-	252	WP_003409899.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FPJ42_RS10150	2188497..2188991	+	495	WP_003899099.1	hypothetical protein	-
FPJ42_RS10155	2189151..2189558	-	408	WP_003409913.1	type II toxin-antitoxin system VapC family toxin	-
FPJ42_RS10160	2189562..2189834	-	273	WP_003899100.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ42_RS10165	2189867..2191087	-	1221	WP_003409919.1	TetR family transcriptional regulator Mce3R	-
FPJ42_RS10170	2191986..2192783	+	798	WP_003899101.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.64 Da
• Isoelectric Point: 6.8604

>T133870 WP_003409896.1 NZ_CP041864:c2188262-2187966 [Mycobacterium tuberculosis]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL

Nucleotide

• Length: 297 bp

>T133870 NZ_CP041864:c2188262-2187966 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9207.17 Da
• Isoelectric Point: 7.0150

>AT133870 WP_003409899.1 NZ_CP041864:c2188510-2188259 [Mycobacterium tuberculosis]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR

Nucleotide

• Length: 252 bp

>AT133870 NZ_CP041864:c2188510-2188259 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TLU9

Antitoxin

• AlphaFold DB: A0A7U4BUZ2