Detailed information of TA system TA133868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2177572..2178275
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ42_RS10040
• Protein ID: WP_003409778.1
• Coordinates: 2177572..2177901 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ42_RS10045
• Protein ID: WP_003409780.1
• Coordinates: 2177898..2178275 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS10020	2173955..2175025	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPJ42_RS10025	2175022..2175537	+	516	WP_003409718.1	flavin reductase family protein	-
FPJ42_RS10030	2175534..2176595	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ42_RS10035	2176592..2177362	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ42_RS10040	2177572..2177901	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ42_RS10045	2177898..2178275	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ42_RS10050	2178272..2178862	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ42_RS10055	2178917..2180280	+	1364	Protein_1986	HNH endonuclease	-
FPJ42_RS10060	2180435..2180887	-	453	WP_003899095.1	lipoprotein	-
FPJ42_RS10065	2180951..2181352	+	402	WP_003409869.1	hypothetical protein	-
FPJ42_RS10070	2181345..2181527	-	183	WP_003409870.1	hypothetical protein	-
FPJ42_RS10075	2181641..2181991	-	351	WP_003899096.1	hypothetical protein	-
FPJ42_RS10080	2182002..2182481	-	480	Protein_1991	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T133868 WP_003409778.1 NZ_CP041864:c2177901-2177572 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T133868 NZ_CP041864:c2177901-2177572 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT133868 WP_003409780.1 NZ_CP041864:c2178275-2177898 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT133868 NZ_CP041864:c2178275-2177898 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8