Detailed information of TA system TA133852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 752157..752949
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ42_RS03475
• Protein ID: WP_003403386.1
• Coordinates: 752512..752949 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ42_RS03470
• Protein ID: WP_003403381.1
• Coordinates: 752157..752402 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS03440	747177..748682	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ42_RS03445	748763..749773	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ42_RS03450	750161..750544	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ42_RS03455	750639..750794	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ42_RS03460	750870..751586	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ42_RS03465	751862..752170	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ42_RS03470	752157..752402	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ42_RS03475	752512..752949	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ42_RS03480	752946..753200	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ42_RS03485	753314..755677	+	2364	WP_003898547.1	arylsulfatase AtsD	-
FPJ42_RS03490	755740..756066	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ42_RS03495	755978..756316	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ42_RS03500	756313..756486	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T133852 WP_003403386.1 NZ_CP041864:c752949-752512 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T133852 NZ_CP041864:c752949-752512 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT133852 WP_003403381.1 NZ_CP041864:c752402-752157 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT133852 NZ_CP041864:c752402-752157 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0