Detailed information of TA system TA133848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 713587..714236
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ42_RS03265
• Protein ID: WP_193093013.1
• Coordinates: 713841..714236 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: FPJ42_RS03260
• Protein ID: WP_003403235.1
• Coordinates: 713587..713841 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS03235	708713..709408	+	696	WP_003900189.1	galactose-1-phosphate uridylyltransferase	-
FPJ42_RS03240	709405..709896	+	492	WP_012054156.1	galactose-1-phosphate uridylyltransferase	-
FPJ42_RS03245	709893..710984	+	1092	WP_003906403.1	galactokinase	-
FPJ42_RS03250	711379..712443	+	1065	WP_003898532.1	hypothetical protein	-
FPJ42_RS03255	712547..713494	+	948	WP_003403232.1	DUF732 domain-containing protein	-
FPJ42_RS03260	713587..713841	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
FPJ42_RS03265	713841..714236	+	396	WP_193093013.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
FPJ42_RS03270	714330..715070	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
FPJ42_RS03275	715202..715462	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ42_RS03280	715459..715866	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
FPJ42_RS03285	715938..717089	-	1152	WP_003403248.1	FIST C-terminal domain-containing protein	-
FPJ42_RS03290	717182..718909	-	1728	WP_193093014.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14220.02 Da
• Isoelectric Point: 4.9319

>T133848 WP_193093013.1 NZ_CP041864:713841-714236 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLRLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T133848 NZ_CP041864:713841-714236 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGCGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT133848 WP_003403235.1 NZ_CP041864:713587-713841 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT133848 NZ_CP041864:713587-713841 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSC2