Detailed information of TA system TA133847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 707959..708584
• Replicon: chromosome
• Accession: NZ_CP041864
• Organism: Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ42_RS03230
• Protein ID: WP_031654475.1
• Coordinates: 708183..708584 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ36
• Locus tag: FPJ42_RS03225
• Protein ID: WP_003403213.1
• Coordinates: 707959..708186 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ42_RS03200	703138..703359	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
FPJ42_RS03205	703501..704106	+	606	WP_003898526.1	hypothetical protein	-
FPJ42_RS03210	704125..706692	-	2568	WP_003900188.1	SEC-C domain-containing protein	-
FPJ42_RS03215	706776..707525	+	750	WP_003898528.1	hypothetical protein	-
FPJ42_RS03220	707522..707764	+	243	WP_003403210.1	hypothetical protein	-
FPJ42_RS03225	707959..708186	+	228	WP_003403213.1	antitoxin VapB29	Antitoxin
FPJ42_RS03230	708183..708584	+	402	WP_031654475.1	type II toxin-antitoxin system ribonuclease VapC29	Toxin
FPJ42_RS03235	708713..709408	+	696	WP_003900189.1	galactose-1-phosphate uridylyltransferase	-
FPJ42_RS03240	709405..709896	+	492	WP_012054156.1	galactose-1-phosphate uridylyltransferase	-
FPJ42_RS03245	709893..710984	+	1092	WP_003906403.1	galactokinase	-
FPJ42_RS03250	711379..712443	+	1065	WP_003898532.1	hypothetical protein	-
FPJ42_RS03255	712547..713494	+	948	WP_003403232.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13953.81 Da
• Isoelectric Point: 5.7441

>T133847 WP_031654475.1 NZ_CP041864:708183-708584 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLILTTT

Nucleotide

• Length: 402 bp

>T133847 NZ_CP041864:708183-708584 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCTAACGACCACCT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8163.17 Da
• Isoelectric Point: 5.6307

>AT133847 WP_003403213.1 NZ_CP041864:707959-708186 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Nucleotide

• Length: 228 bp

>AT133847 NZ_CP041864:707959-708186 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSC9