Detailed information of TA system TA133769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3154533..3155137
• Replicon: chromosome
• Accession: NZ_CP041862
• Organism: Mycobacterium tuberculosis strain 1-0047P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FPJ44_RS14875
• Protein ID: WP_003414492.1
• Coordinates: 3154533..3154925 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FPJ44_RS14880
• Protein ID: WP_003414495.1
• Coordinates: 3154922..3155137 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ44_RS14845	3149683..3150471	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPJ44_RS14850	3150805..3151350	-	546	WP_019284034.1	DUF1802 family protein	-
FPJ44_RS14855	3151622..3152506	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FPJ44_RS14860	3152509..3153396	-	888	WP_015630563.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FPJ44_RS14865	3153701..3154246	-	546	WP_019284034.1	DUF1802 family protein	-
FPJ44_RS14870	3154243..3154512	-	270	WP_003414489.1	DUF2277 family protein	-
FPJ44_RS14875	3154533..3154925	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ44_RS14880	3154922..3155137	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPJ44_RS14885	3155184..3155933	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FPJ44_RS14890	3156012..3157094	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
FPJ44_RS14895	3157087..3158397	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FPJ44_RS14900	3158400..3159227	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FPJ44_RS14905	3159224..3160135	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3135696..3156390	20694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T133769 WP_003414492.1 NZ_CP041862:c3154925-3154533 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T133769 NZ_CP041862:c3154925-3154533 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT133769 WP_003414495.1 NZ_CP041862:c3155137-3154922 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT133769 NZ_CP041862:c3155137-3154922 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8