Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3127879..3128449 Replicon chromosome
Accession NZ_CP041862
Organism Mycobacterium tuberculosis strain 1-0047P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ44_RS14730 Protein ID WP_003414166.1
Coordinates 3127879..3128235 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ44_RS14735 Protein ID WP_003901465.1
Coordinates 3128219..3128449 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ44_RS14710 3123331..3125019 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ44_RS14715 3125023..3125349 - 327 WP_003414157.1 hypothetical protein -
FPJ44_RS14720 3125522..3126109 + 588 WP_003914429.1 DUF3558 family protein -
FPJ44_RS14725 3126128..3127777 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ44_RS14730 3127879..3128235 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ44_RS14735 3128219..3128449 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ44_RS14740 3128492..3129535 - 1044 WP_016720604.1 DUF2293 domain-containing protein -
FPJ44_RS14745 3129726..3130001 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ44_RS14750 3130177..3130431 - 255 WP_155936531.1 hypothetical protein -
FPJ44_RS14755 3130579..3130983 + 405 WP_003414181.1 hypothetical protein -
FPJ44_RS14760 3130980..3131171 + 192 WP_003414184.1 hypothetical protein -
FPJ44_RS14765 3131235..3132523 + 1289 Protein_2914 transposase family protein -
FPJ44_RS14770 3132757..3133014 + 258 WP_003899489.1 hypothetical protein -
FPJ44_RS14775 3133119..3133430 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T133767 WP_003414166.1 NZ_CP041862:c3128235-3127879 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T133767 NZ_CP041862:c3128235-3127879 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT133767 WP_003901465.1 NZ_CP041862:c3128449-3128219 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT133767 NZ_CP041862:c3128449-3128219 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References