Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3106600..3107170 Replicon chromosome
Accession NZ_CP041861
Organism Mycobacterium tuberculosis strain 1-0054P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ45_RS14645 Protein ID WP_003414166.1
Coordinates 3106600..3106956 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ45_RS14650 Protein ID WP_003901465.1
Coordinates 3106940..3107170 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ45_RS14625 3102052..3103740 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ45_RS14630 3103744..3104070 - 327 WP_003414157.1 hypothetical protein -
FPJ45_RS14635 3104243..3104830 + 588 WP_003914429.1 DUF3558 family protein -
FPJ45_RS14640 3104849..3106498 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ45_RS14645 3106600..3106956 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ45_RS14650 3106940..3107170 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ45_RS14655 3107213..3108256 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ45_RS14660 3108447..3108722 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ45_RS14665 3108898..3109152 - 255 WP_003917684.1 hypothetical protein -
FPJ45_RS14670 3109300..3109704 + 405 WP_003414181.1 hypothetical protein -
FPJ45_RS14675 3109701..3109892 + 192 WP_003414184.1 hypothetical protein -
FPJ45_RS14680 3109956..3111245 + 1290 Protein_2897 transposase family protein -
FPJ45_RS14685 3111479..3111736 + 258 WP_003899489.1 hypothetical protein -
FPJ45_RS14690 3111841..3112152 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T133708 WP_003414166.1 NZ_CP041861:c3106956-3106600 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T133708 NZ_CP041861:c3106956-3106600 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT133708 WP_003901465.1 NZ_CP041861:c3107170-3106940 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT133708 NZ_CP041861:c3107170-3106940 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References