Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2867306..2867943 Replicon chromosome
Accession NZ_CP041861
Organism Mycobacterium tuberculosis strain 1-0054P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPJ45_RS13340 Protein ID WP_003413180.1
Coordinates 2867306..2867701 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPJ45_RS13345 Protein ID WP_003413183.1
Coordinates 2867698..2867943 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ45_RS13300 2862711..2863921 + 1211 Protein_2625 alpha/beta hydrolase family protein -
FPJ45_RS13305 2864048..2864707 + 660 WP_003902296.1 hypothetical protein -
FPJ45_RS13310 2864704..2865366 + 663 WP_003905878.1 hypothetical protein -
FPJ45_RS13315 2865363..2865640 + 278 Protein_2628 type II toxin-antitoxin system VapB family antitoxin -
FPJ45_RS13320 2865733..2866146 + 414 WP_003413164.1 PIN domain nuclease -
FPJ45_RS13325 2866185..2866442 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPJ45_RS13330 2866439..2866816 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPJ45_RS13335 2866832..2867206 - 375 WP_003413177.1 hypothetical protein -
FPJ45_RS13340 2867306..2867701 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPJ45_RS13345 2867698..2867943 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPJ45_RS13350 2868354..2868773 - 420 WP_003413190.1 A24 family peptidase -
FPJ45_RS13355 2868785..2869594 - 810 WP_003413193.1 shikimate dehydrogenase -
FPJ45_RS13360 2869591..2870844 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPJ45_RS13365 2870837..2871349 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T133703 WP_003413180.1 NZ_CP041861:c2867701-2867306 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T133703 NZ_CP041861:c2867701-2867306 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT133703 WP_003413183.1 NZ_CP041861:c2867943-2867698 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT133703 NZ_CP041861:c2867943-2867698 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References