Detailed information of TA system TA133675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 839133..839842
• Replicon: chromosome
• Accession: NZ_CP041861
• Organism: Mycobacterium tuberculosis strain 1-0054P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ45_RS03925
• Protein ID: WP_116298772.1
• Coordinates: 839435..839842 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: FPJ45_RS03920
• Protein ID: WP_003403834.1
• Coordinates: 839133..839390 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ45_RS03915	836769..839042	+	2274	WP_016330384.1	PE family protein	-
FPJ45_RS03920	839133..839390	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ45_RS03925	839435..839842	+	408	WP_116298772.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ45_RS03930	839932..840106	-	175	Protein_777	transposase	-
FPJ45_RS03935	840219..840464	+	246	WP_003403841.1	hypothetical protein	-
FPJ45_RS03940	840533..841417	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
FPJ45_RS03945	841428..842590	-	1163	Protein_780	acyl-CoA dehydrogenase family protein	-
FPJ45_RS03950	842597..844129	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15026.31 Da
• Isoelectric Point: 8.0185

>T133675 WP_116298772.1 NZ_CP041861:839435-839842 [Mycobacterium tuberculosis]
VLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHLPTNPGPR
HLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 408 bp

>T133675 NZ_CP041861:839435-839842 [Mycobacterium tuberculosis]
GTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTTCGATCGACTGCTCGCGGCTGA
CGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATCGACGCATCTTCGAGATTCCGT
CACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTTCCGACGAACCCCGGTCCCAGA
CACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACCTGACGCGGTACTCGCGGCCAT
AGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCTCGGTGCGCCACATTCGCCCGC
CGCTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT133675 WP_003403834.1 NZ_CP041861:839133-839390 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT133675 NZ_CP041861:839133-839390 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Antitoxin

• AlphaFold DB: G0TEX4