Detailed information of TA system TA133659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 360978..361504
• Replicon: chromosome
• Accession: NZ_CP041861
• Organism: Mycobacterium tuberculosis strain 1-0054P6C4

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FPJ45_RS01565
• Protein ID: WP_003401560.1
• Coordinates: 361202..361504 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: FPJ45_RS01560
• Protein ID: WP_003401555.1
• Coordinates: 360978..361205 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ45_RS01545	356671..357474	-	804	WP_003401540.1	hypothetical protein	-
FPJ45_RS01550	357484..358881	-	1398	WP_003401544.1	sulfatase	-
FPJ45_RS01555	359060..360835	+	1776	WP_010886074.1	PE family protein	-
FPJ45_RS01560	360978..361205	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ45_RS01565	361202..361504	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FPJ45_RS01570	361552..361773	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
FPJ45_RS01575	361770..362195	+	426	WP_003401566.1	PIN domain nuclease	-
FPJ45_RS01580	362331..362963	+	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
FPJ45_RS01585	362960..363868	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T133659 WP_003401560.1 NZ_CP041861:361202-361504 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T133659 NZ_CP041861:361202-361504 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT133659 WP_003401555.1 NZ_CP041861:360978-361205 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT133659 NZ_CP041861:360978-361205 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1