Detailed information of TA system TA133652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3175019..3175706
• Replicon: chromosome
• Accession: NZ_CP041860
• Organism: Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FPJ46_RS15005
• Protein ID: WP_003414624.1
• Coordinates: 3175263..3175706 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: FPJ46_RS15000
• Protein ID: WP_003414620.1
• Coordinates: 3175019..3175276 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ46_RS14980	3170339..3171193	-	855	WP_031676248.1	GNAT family N-acetyltransferase	-
FPJ46_RS14985	3171249..3172412	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPJ46_RS14990	3172429..3173643	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FPJ46_RS14995	3173651..3174892	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FPJ46_RS15000	3175019..3175276	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ46_RS15005	3175263..3175706	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ46_RS15010	3175786..3176448	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FPJ46_RS15015	3176544..3176735	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ46_RS15020	3177067..3178815	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FPJ46_RS15025	3178911..3179492	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FPJ46_RS15030	3179592..3179858	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T133652 WP_003414624.1 NZ_CP041860:3175263-3175706 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T133652 NZ_CP041860:3175263-3175706 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT133652 WP_003414620.1 NZ_CP041860:3175019-3175276 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT133652 NZ_CP041860:3175019-3175276 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0