Detailed information of TA system TA133651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3169418..3169966
• Replicon: chromosome
• Accession: NZ_CP041860
• Organism: Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: FPJ46_RS14975
• Protein ID: WP_003414602.1
• Coordinates: 3169703..3169966 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: FPJ46_RS14970
• Protein ID: WP_003414599.1
• Coordinates: 3169418..3169699 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ46_RS14945	3165042..3165899	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
FPJ46_RS14950	3165941..3166525	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
FPJ46_RS14955	3166628..3166876	+	249	WP_003913411.1	antitoxin VapB23	-
FPJ46_RS14960	3166873..3167253	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
FPJ46_RS14965	3167335..3169146	-	1812	WP_003414596.1	penicillin-binding protein	-
FPJ46_RS14970	3169418..3169699	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
FPJ46_RS14975	3169703..3169966	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ46_RS14980	3170339..3171193	-	855	WP_031676248.1	GNAT family N-acetyltransferase	-
FPJ46_RS14985	3171249..3172412	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPJ46_RS14990	3172429..3173643	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FPJ46_RS14995	3173651..3174892	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T133651 WP_003414602.1 NZ_CP041860:3169703-3169966 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T133651 NZ_CP041860:3169703-3169966 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT133651 WP_003414599.1 NZ_CP041860:3169418-3169699 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT133651 NZ_CP041860:3169418-3169699 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8