Detailed information of TA system TA133642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2867992..2868623
• Replicon: chromosome
• Accession: NZ_CP041860
• Organism: Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ46_RS13335
• Protein ID: WP_003413174.1
• Coordinates: 2868246..2868623 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ46_RS13330
• Protein ID: WP_003413167.1
• Coordinates: 2867992..2868249 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ46_RS13300	2863907..2864221	+	315	WP_009937839.1	hypothetical protein	-
FPJ46_RS13305	2864517..2865728	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ46_RS13310	2865855..2866514	+	660	WP_003902296.1	hypothetical protein	-
FPJ46_RS13315	2866511..2867173	+	663	WP_031729659.1	hypothetical protein	-
FPJ46_RS13320	2867170..2867447	+	278	Protein_2629	type II toxin-antitoxin system VapB family antitoxin	-
FPJ46_RS13325	2867540..2867953	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ46_RS13330	2867992..2868249	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ46_RS13335	2868246..2868623	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ46_RS13340	2868639..2869013	-	375	WP_003413177.1	hypothetical protein	-
FPJ46_RS13345	2869113..2869508	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ46_RS13350	2869505..2869750	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ46_RS13355	2870161..2870580	-	420	WP_003413190.1	A24 family peptidase	-
FPJ46_RS13360	2870592..2871401	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ46_RS13365	2871398..2872651	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ46_RS13370	2872644..2873156	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T133642 WP_003413174.1 NZ_CP041860:2868246-2868623 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T133642 NZ_CP041860:2868246-2868623 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT133642 WP_003413167.1 NZ_CP041860:2867992-2868249 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT133642 NZ_CP041860:2867992-2868249 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5