Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2228287..2228873 Replicon chromosome
Accession NZ_CP041860
Organism Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FPJ46_RS10350 Protein ID WP_003410010.1
Coordinates 2228287..2228631 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FPJ46_RS10355 Protein ID WP_003410014.1
Coordinates 2228625..2228873 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ46_RS10315 2223993..2224592 + 600 WP_003910883.1 L-lysine exporter -
FPJ46_RS10320 2225008..2225436 + 429 WP_003409992.1 cellulose-binding protein -
FPJ46_RS10325 2225662..2226201 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FPJ46_RS10330 2226721..2227281 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FPJ46_RS10335 2227278..2227619 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FPJ46_RS10340 2227705..2227962 + 258 WP_003410006.1 hypothetical protein -
FPJ46_RS10345 2227863..2228198 - 336 WP_003410009.1 dehydrogenase -
FPJ46_RS10350 2228287..2228631 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FPJ46_RS10355 2228625..2228873 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FPJ46_RS10360 2228973..2231288 - 2316 WP_003899120.1 cation transporter ATPase CptG -
FPJ46_RS10365 2231285..2231557 - 273 WP_003410017.1 DUF1490 family protein -
FPJ46_RS10370 2231610..2231966 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FPJ46_RS10375 2232123..2232890 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T133633 WP_003410010.1 NZ_CP041860:c2228631-2228287 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T133633 NZ_CP041860:c2228631-2228287 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT133633 WP_003410014.1 NZ_CP041860:c2228873-2228625 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT133633 NZ_CP041860:c2228873-2228625 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References