Detailed information of TA system TA133625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1769493..1770106
• Replicon: chromosome
• Accession: NZ_CP041860
• Organism: Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: FPJ46_RS08205
• Protein ID: WP_003407786.1
• Coordinates: 1769702..1770106 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FPJ46_RS08200
• Protein ID: WP_009935474.1
• Coordinates: 1769493..1769696 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ46_RS08170	1764898..1765278	+	381	WP_003407768.1	fumarate reductase subunit C	-
FPJ46_RS08175	1765275..1765652	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
FPJ46_RS08180	1765720..1766328	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
FPJ46_RS08185	1766512..1767660	+	1149	Protein_1614	MMPL family transporter	-
FPJ46_RS08190	1767670..1768116	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FPJ46_RS08195	1768151..1769440	+	1290	WP_003407781.1	threonine ammonia-lyase	-
FPJ46_RS08200	1769493..1769696	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ46_RS08205	1769702..1770106	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FPJ46_RS08210	1770123..1771865	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
FPJ46_RS08215	1771858..1774155	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T133625 WP_003407786.1 NZ_CP041860:1769702-1770106 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T133625 NZ_CP041860:1769702-1770106 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT133625 WP_009935474.1 NZ_CP041860:1769493-1769696 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT133625 NZ_CP041860:1769493-1769696 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46