Detailed information of TA system TA133546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701204..701850
• Replicon: chromosome
• Accession: NZ_CP041859
• Organism: Mycobacterium tuberculosis strain 1-0061P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FPJ47_RS03125
• Protein ID: WP_003403137.1
• Coordinates: 701204..701617 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FPJ47_RS03130
• Protein ID: WP_003403139.1
• Coordinates: 701614..701850 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ47_RS03105	697287..698837	+	1551	WP_003403119.1	MCE family protein	-
FPJ47_RS03110	698889..699281	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FPJ47_RS03115	699278..699535	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FPJ47_RS03120	699718..700953	-	1236	WP_193061966.1	ATP-binding protein	-
FPJ47_RS03125	701204..701617	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ47_RS03130	701614..701850	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FPJ47_RS03135	701954..702460	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FPJ47_RS03140	702574..703104	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPJ47_RS03145	703088..703849	-	762	WP_003904438.1	two-component system response regulator TcrA	-
FPJ47_RS03150	703906..704217	+	312	WP_003403164.1	hypothetical protein	-
FPJ47_RS03155	704289..705239	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
FPJ47_RS03160	705480..706064	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FPJ47_RS03165	706066..706776	+	711	Protein_627	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T133546 WP_003403137.1 NZ_CP041859:c701617-701204 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T133546 NZ_CP041859:c701617-701204 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT133546 WP_003403139.1 NZ_CP041859:c701850-701614 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT133546 NZ_CP041859:c701850-701614 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89