Detailed information of TA system TA133527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3066486..3067173
• Replicon: chromosome
• Accession: NZ_CP041858
• Organism: Mycobacterium tuberculosis strain 1-0064P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FPJ48_RS14435
• Protein ID: WP_003414064.1
• Coordinates: 3066778..3067173 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ48_RS14430
• Protein ID: WP_003414061.1
• Coordinates: 3066486..3066752 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ48_RS14405	3062125..3063027	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ48_RS14410	3063096..3063848	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ48_RS14415	3064092..3064367	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ48_RS14420	3064364..3065986	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ48_RS14425	3066073..3066489	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FPJ48_RS14430	3066486..3066752	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ48_RS14435	3066778..3067173	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ48_RS14440	3067170..3067439	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ48_RS14445	3067449..3068543	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ48_RS14450	3068540..3068959	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ48_RS14455	3069033..3069512	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ48_RS14460	3069583..3070383	-	801	WP_003911953.1	thymidylate synthase	-
FPJ48_RS14465	3070539..3071276	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T133527 WP_003414064.1 NZ_CP041858:c3067173-3066778 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T133527 NZ_CP041858:c3067173-3066778 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT133527 WP_003414061.1 NZ_CP041858:c3066752-3066486 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT133527 NZ_CP041858:c3066752-3066486 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64