Detailed information of TA system TA133523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2866741..2867378
• Replicon: chromosome
• Accession: NZ_CP041858
• Organism: Mycobacterium tuberculosis strain 1-0064P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ48_RS13340
• Protein ID: WP_003413180.1
• Coordinates: 2866741..2867136 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPJ48_RS13345
• Protein ID: WP_003413183.1
• Coordinates: 2867133..2867378 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ48_RS13300	2862145..2863356	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ48_RS13305	2863483..2864142	+	660	WP_003902296.1	hypothetical protein	-
FPJ48_RS13310	2864139..2864801	+	663	WP_003905878.1	hypothetical protein	-
FPJ48_RS13315	2864798..2865075	+	278	Protein_2628	type II toxin-antitoxin system VapB family antitoxin	-
FPJ48_RS13320	2865168..2865581	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ48_RS13325	2865620..2865877	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ48_RS13330	2865874..2866251	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ48_RS13335	2866267..2866641	-	375	WP_003413177.1	hypothetical protein	-
FPJ48_RS13340	2866741..2867136	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ48_RS13345	2867133..2867378	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ48_RS13350	2867789..2868208	-	420	WP_003413190.1	A24 family peptidase	-
FPJ48_RS13355	2868220..2869029	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ48_RS13360	2869026..2870279	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ48_RS13365	2870272..2870784	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T133523 WP_003413180.1 NZ_CP041858:c2867136-2866741 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T133523 NZ_CP041858:c2867136-2866741 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT133523 WP_003413183.1 NZ_CP041858:c2867378-2867133 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT133523 NZ_CP041858:c2867378-2867133 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31