Detailed information of TA system TA133493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 753871..754663
• Replicon: chromosome
• Accession: NZ_CP041858
• Organism: Mycobacterium tuberculosis strain 1-0064P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ48_RS03460
• Protein ID: WP_003403386.1
• Coordinates: 754226..754663 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ48_RS03455
• Protein ID: WP_003403381.1
• Coordinates: 753871..754116 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ48_RS03425	748891..750396	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ48_RS03430	750477..751487	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ48_RS03435	751875..752258	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ48_RS03440	752353..752508	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ48_RS03445	752584..753300	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ48_RS03450	753576..753884	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ48_RS03455	753871..754116	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ48_RS03460	754226..754663	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ48_RS03465	754660..754914	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ48_RS03470	755028..757391	+	2364	WP_003901895.1	arylsulfatase AtsD	-
FPJ48_RS03475	757454..757780	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ48_RS03480	757692..758030	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ48_RS03485	758027..758200	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T133493 WP_003403386.1 NZ_CP041858:c754663-754226 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T133493 NZ_CP041858:c754663-754226 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT133493 WP_003403381.1 NZ_CP041858:c754116-753871 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT133493 NZ_CP041858:c754116-753871 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0