Detailed information of TA system TA133280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2853306..2853946
• Replicon: chromosome
• Accession: NZ_CP041854
• Organism: Mycobacterium tuberculosis strain 1-0102P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FPJ54_RS13260
• Protein ID: WP_003412970.1
• Coordinates: 2853306..2853725 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FPJ54_RS13265
• Protein ID: WP_003412975.1
• Coordinates: 2853722..2853946 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ54_RS13230	2848896..2849618	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FPJ54_RS13235	2850135..2850357	+	223	Protein_2612	type II toxin-antitoxin system VapB family antitoxin	-
FPJ54_RS13240	2850354..2850755	+	402	WP_003412963.1	PIN domain-containing protein	-
FPJ54_RS13245	2850790..2851710	-	921	WP_003412965.1	restriction endonuclease	-
FPJ54_RS13250	2852051..2852296	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ54_RS13255	2852355..2853305	+	951	WP_003911916.1	Lsr2 family protein	-
FPJ54_RS13260	2853306..2853725	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ54_RS13265	2853722..2853946	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FPJ54_RS13270	2853977..2856820	-	2844	WP_193057565.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FPJ54_RS13275	2856892..2857293	-	402	WP_003412981.1	hypothetical protein	-
FPJ54_RS13280	2857293..2857763	-	471	WP_003899364.1	transcription antitermination factor NusB	-
FPJ54_RS13285	2857766..2858329	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T133280 WP_003412970.1 NZ_CP041854:c2853725-2853306 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T133280 NZ_CP041854:c2853725-2853306 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT133280 WP_003412975.1 NZ_CP041854:c2853946-2853722 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT133280 NZ_CP041854:c2853946-2853722 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09