Detailed information of TA system TA133274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2253840..2254466
• Replicon: chromosome
• Accession: NZ_CP041854
• Organism: Mycobacterium tuberculosis strain 1-0102P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPJ54_RS10490
• Protein ID: WP_003410075.1
• Coordinates: 2254068..2254466 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPJ54_RS10485
• Protein ID: WP_003911750.1
• Coordinates: 2253840..2254067 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ54_RS10475	2251879..2252223	-	345	WP_003410065.1	ferredoxin family protein	-
FPJ54_RS10480	2252412..2253581	-	1170	WP_003899126.1	ATP-binding protein	-
FPJ54_RS10485	2253840..2254067	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ54_RS10490	2254068..2254466	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FPJ54_RS10495	2254649..2255080	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPJ54_RS10500	2255181..2255615	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPJ54_RS10505	2256052..2256231	-	180	Protein_2076	hypothetical protein	-
FPJ54_RS10510	2256292..2256939	+	648	WP_071854215.1	transposase	-
FPJ54_RS10515	2256893..2257483	+	591	WP_003899131.1	IS110 family transposase	-
FPJ54_RS10520	2257611..2258867	-	1257	WP_003902247.1	HNH endonuclease	-
FPJ54_RS10525	2259221..2259421	+	201	Protein_2080	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T133274 WP_003410075.1 NZ_CP041854:2254068-2254466 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T133274 NZ_CP041854:2254068-2254466 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT133274 WP_003911750.1 NZ_CP041854:2253840-2254067 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT133274 NZ_CP041854:2253840-2254067 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8