Detailed information of TA system TA133255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 840481..841190
• Replicon: chromosome
• Accession: NZ_CP041854
• Organism: Mycobacterium tuberculosis strain 1-0102P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ54_RS03940
• Protein ID: WP_116298772.1
• Coordinates: 840783..841190 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: FPJ54_RS03935
• Protein ID: WP_003403834.1
• Coordinates: 840481..840738 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ54_RS03930	838117..840390	+	2274	WP_016330384.1	PE family protein	-
FPJ54_RS03935	840481..840738	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ54_RS03940	840783..841190	+	408	WP_116298772.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ54_RS03945	841280..841454	-	175	Protein_780	transposase	-
FPJ54_RS03950	841567..841812	+	246	WP_003403841.1	hypothetical protein	-
FPJ54_RS03955	841881..842765	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
FPJ54_RS03960	842776..843938	-	1163	Protein_783	acyl-CoA dehydrogenase family protein	-
FPJ54_RS03965	843945..845477	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15026.31 Da
• Isoelectric Point: 8.0185

>T133255 WP_116298772.1 NZ_CP041854:840783-841190 [Mycobacterium tuberculosis]
VLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHLPTNPGPR
HLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 408 bp

>T133255 NZ_CP041854:840783-841190 [Mycobacterium tuberculosis]
GTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTTCGATCGACTGCTCGCGGCTGA
CGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATCGACGCATCTTCGAGATTCCGT
CACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTTCCGACGAACCCCGGTCCCAGA
CACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACCTGACGCGGTACTCGCGGCCAT
AGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCTCGGTGCGCCACATTCGCCCGC
CGCTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT133255 WP_003403834.1 NZ_CP041854:840481-840738 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT133255 NZ_CP041854:840481-840738 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Antitoxin

• AlphaFold DB: G0TEX4