Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2258768..2259354 Replicon chromosome
Accession NZ_CP041853
Organism Mycobacterium tuberculosis strain 1-0107P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FPJ55_RS10510 Protein ID WP_003410010.1
Coordinates 2258768..2259112 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FPJ55_RS10515 Protein ID WP_003410014.1
Coordinates 2259106..2259354 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ55_RS10475 2254474..2255073 + 600 WP_003409989.1 L-lysine exporter -
FPJ55_RS10480 2255489..2255917 + 429 WP_003409992.1 cellulose-binding protein -
FPJ55_RS10485 2256143..2256682 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FPJ55_RS10490 2257202..2257762 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FPJ55_RS10495 2257759..2258100 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FPJ55_RS10500 2258186..2258443 + 258 WP_003410006.1 hypothetical protein -
FPJ55_RS10505 2258344..2258679 - 336 WP_003410009.1 dehydrogenase -
FPJ55_RS10510 2258768..2259112 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FPJ55_RS10515 2259106..2259354 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FPJ55_RS10520 2259454..2261706 - 2253 WP_016719189.1 cation transporter ATPase CptG -
FPJ55_RS10525 2261703..2261975 - 273 WP_003410017.1 DUF1490 family protein -
FPJ55_RS10530 2262028..2262384 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FPJ55_RS10535 2262541..2263308 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T133213 WP_003410010.1 NZ_CP041853:c2259112-2258768 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T133213 NZ_CP041853:c2259112-2258768 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT133213 WP_003410014.1 NZ_CP041853:c2259354-2259106 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT133213 NZ_CP041853:c2259354-2259106 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References