Detailed information of TA system TA133166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3067128..3067807
• Replicon: chromosome
• Accession: NZ_CP041852
• Organism: Mycobacterium tuberculosis strain 1-0110P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ56_RS14415
• Protein ID: WP_003414059.1
• Coordinates: 3067128..3067544 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ56_RS14420
• Protein ID: WP_003414061.1
• Coordinates: 3067541..3067807 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ56_RS14395	3063180..3064082	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ56_RS14400	3064151..3064903	-	753	WP_193056865.1	FAD-dependent thymidylate synthase	-
FPJ56_RS14405	3065147..3065422	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ56_RS14410	3065419..3067041	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ56_RS14415	3067128..3067544	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ56_RS14420	3067541..3067807	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ56_RS14425	3067833..3068228	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ56_RS14430	3068225..3068494	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ56_RS14435	3068504..3068767	-	264	Protein_2848	restriction endonuclease subunit S	-
FPJ56_RS14440	3068886..3069116	+	231	Protein_2849	winged helix-turn-helix domain-containing protein	-
FPJ56_RS14445	3069425..3070573	-	1149	WP_003414088.1	PPE family protein	-
FPJ56_RS14450	3070697..3071149	-	453	WP_003414091.1	flavodoxin family protein	-
FPJ56_RS14455	3071235..3071708	-	474	WP_003414097.1	hypothetical protein	-
FPJ56_RS14460	3071720..3072457	-	738	WP_003414099.1	4-hydroxy-tetrahydrodipicolinate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T133166 WP_003414059.1 NZ_CP041852:c3067544-3067128 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T133166 NZ_CP041852:c3067544-3067128 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT133166 WP_003414061.1 NZ_CP041852:c3067807-3067541 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT133166 NZ_CP041852:c3067807-3067541 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64