Detailed information of TA system TA133036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2250226..2250852
• Replicon: chromosome
• Accession: NZ_CP041850
• Organism: Mycobacterium tuberculosis strain 1-0116P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPJ58_RS10455
• Protein ID: WP_003410075.1
• Coordinates: 2250454..2250852 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPJ58_RS10450
• Protein ID: WP_003911750.1
• Coordinates: 2250226..2250453 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ58_RS10440	2248265..2248609	-	345	WP_003410065.1	ferredoxin family protein	-
FPJ58_RS10445	2248798..2249967	-	1170	WP_003899126.1	ATP-binding protein	-
FPJ58_RS10450	2250226..2250453	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ58_RS10455	2250454..2250852	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FPJ58_RS10460	2251035..2251466	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPJ58_RS10465	2251567..2252001	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPJ58_RS10470	2252438..2252617	-	180	Protein_2069	hypothetical protein	-
FPJ58_RS10475	2252678..2253325	+	648	WP_071854215.1	transposase	-
FPJ58_RS10480	2253279..2253869	+	591	WP_003899131.1	IS110 family transposase	-
FPJ58_RS10485	2253997..2255253	-	1257	WP_003902247.1	HNH endonuclease	-
FPJ58_RS10490	2255607..2255807	+	201	Protein_2073	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T133036 WP_003410075.1 NZ_CP041850:2250454-2250852 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T133036 NZ_CP041850:2250454-2250852 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT133036 WP_003911750.1 NZ_CP041850:2250226-2250453 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT133036 NZ_CP041850:2250226-2250453 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8