Detailed information of TA system TA132750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3118902..3119472
• Replicon: chromosome
• Accession: NZ_CP041845
• Organism: Mycobacterium tuberculosis strain 1-0156P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ63_RS14700
• Protein ID: WP_003414166.1
• Coordinates: 3118902..3119258 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ63_RS14705
• Protein ID: WP_003901465.1
• Coordinates: 3119242..3119472 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ63_RS14680	3114354..3116042	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FPJ63_RS14685	3116046..3116372	-	327	WP_003414157.1	hypothetical protein	-
FPJ63_RS14690	3116545..3117132	+	588	WP_003914429.1	DUF3558 family protein	-
FPJ63_RS14695	3117151..3118800	+	1650	WP_031654722.1	CocE/NonD family hydrolase	-
FPJ63_RS14700	3118902..3119258	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ63_RS14705	3119242..3119472	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ63_RS14710	3119515..3120558	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ63_RS14715	3120749..3121024	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
FPJ63_RS14720	3121200..3121454	-	255	WP_003917684.1	hypothetical protein	-
FPJ63_RS14725	3121602..3122006	+	405	WP_009938577.1	hypothetical protein	-
FPJ63_RS14730	3122003..3122194	+	192	WP_003414184.1	hypothetical protein	-
FPJ63_RS14735	3122258..3123547	+	1290	Protein_2908	transposase family protein	-
FPJ63_RS14740	3123781..3124038	+	258	WP_003899489.1	hypothetical protein	-
FPJ63_RS14745	3124143..3124454	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3108937..3133700	24763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T132750 WP_003414166.1 NZ_CP041845:c3119258-3118902 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T132750 NZ_CP041845:c3119258-3118902 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT132750 WP_003901465.1 NZ_CP041845:c3119472-3119242 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT132750 NZ_CP041845:c3119472-3119242 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3