Detailed information of TA system TA132656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1020167..1020786
• Replicon: chromosome
• Accession: NZ_CP041844
• Organism: Mycobacterium tuberculosis strain 1-0160P6C4

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FPJ64_RS04800
• Protein ID: WP_031744920.1
• Coordinates: 1020352..1020786 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FPJ64_RS04795
• Protein ID: WP_003898641.1
• Coordinates: 1020167..1020346 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ64_RS04780	1015640..1017220	+	1581	WP_172643130.1	serine hydrolase	-
FPJ64_RS04785	1017217..1019610	+	2394	WP_016719446.1	cation-translocating P-type ATPase	-
FPJ64_RS04790	1019883..1020041	+	159	WP_003404720.1	hypothetical protein	-
FPJ64_RS04795	1020167..1020346	+	180	WP_003898641.1	antitoxin	Antitoxin
FPJ64_RS04800	1020352..1020786	+	435	WP_031744920.1	SRPBCC family protein	Toxin
FPJ64_RS04805	1020884..1021657	+	774	WP_003404735.1	VOC family protein	-
FPJ64_RS04810	1021722..1022171	+	450	WP_003404738.1	hypothetical protein	-
FPJ64_RS04815	1022306..1022536	-	231	WP_003898642.1	hypothetical protein	-
FPJ64_RS04820	1022703..1024211	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FPJ64_RS04825	1024213..1025451	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15768.50 Da
• Isoelectric Point: 9.7977

>T132656 WP_031744920.1 NZ_CP041844:1020352-1020786 [Mycobacterium tuberculosis]
MAKLSGSIDIPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T132656 NZ_CP041844:1020352-1020786 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACATACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT132656 WP_003898641.1 NZ_CP041844:1020167-1020346 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT132656 NZ_CP041844:1020167-1020346 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5