Detailed information of TA system TA132623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2867174..2867805
• Replicon: chromosome
• Accession: NZ_CP041843
• Organism: Mycobacterium tuberculosis strain 1-0168P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ65_RS13325
• Protein ID: WP_003413174.1
• Coordinates: 2867428..2867805 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ65_RS13320
• Protein ID: WP_003413167.1
• Coordinates: 2867174..2867431 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ65_RS13290	2863088..2863402	+	315	WP_009937839.1	hypothetical protein	-
FPJ65_RS13295	2863698..2864909	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ65_RS13300	2865036..2865695	+	660	WP_014585545.1	LppA family lipoprotein	-
FPJ65_RS13305	2865692..2866354	+	663	WP_003900848.1	hypothetical protein	-
FPJ65_RS13310	2866351..2866629	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ65_RS13315	2866722..2867135	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ65_RS13320	2867174..2867431	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ65_RS13325	2867428..2867805	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ65_RS13330	2867821..2868195	-	375	WP_003413177.1	hypothetical protein	-
FPJ65_RS13335	2868295..2868690	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ65_RS13340	2868687..2868932	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ65_RS13345	2869343..2869762	-	420	WP_003413190.1	A24 family peptidase	-
FPJ65_RS13350	2869774..2870583	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ65_RS13355	2870580..2871833	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ65_RS13360	2871826..2872338	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T132623 WP_003413174.1 NZ_CP041843:2867428-2867805 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T132623 NZ_CP041843:2867428-2867805 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT132623 WP_003413167.1 NZ_CP041843:2867174-2867431 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT132623 NZ_CP041843:2867174-2867431 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5