Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1231181..1231728 Replicon chromosome
Accession NZ_CP041843
Organism Mycobacterium tuberculosis strain 1-0168P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag FPJ65_RS05815 Protein ID WP_003898728.1
Coordinates 1231181..1231492 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q8VK70
Locus tag FPJ65_RS05820 Protein ID WP_003913656.1
Coordinates 1231492..1231728 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ65_RS05795 1226662..1228086 - 1425 WP_003405805.1 class II fumarate hydratase -
FPJ65_RS05800 1228117..1229205 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
FPJ65_RS05805 1229261..1229905 + 645 WP_003915494.1 DUF4245 domain-containing protein -
FPJ65_RS05810 1229912..1231069 - 1158 WP_003898727.1 AI-2E family transporter -
FPJ65_RS05815 1231181..1231492 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
FPJ65_RS05820 1231492..1231728 - 237 WP_003913656.1 hypothetical protein Antitoxin
FPJ65_RS05825 1231821..1233346 + 1526 Protein_1147 carboxylesterase/lipase family protein -
FPJ65_RS05830 1233364..1234476 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
FPJ65_RS05835 1234486..1234743 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
FPJ65_RS05840 1234733..1235980 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
FPJ65_RS05845 1235977..1236615 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T132599 WP_003898728.1 NZ_CP041843:c1231492-1231181 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T132599 NZ_CP041843:c1231492-1231181 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8608.66 Da        Isoelectric Point: 5.6908

>AT132599 WP_003913656.1 NZ_CP041843:c1231728-1231492 [Mycobacterium tuberculosis]
VSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEILATNTSATGDLDTLAGHCARTALDID

Download         Length: 237 bp

>AT132599 NZ_CP041843:c1231728-1231492 [Mycobacterium tuberculosis]
GTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATGCCCG
TAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATCCTCG
CCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB Q8VK70

References