Detailed information of TA system TA132537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1022565..1023538
• Replicon: chromosome
• Accession: NZ_CP041842
• Organism: Mycobacterium tuberculosis strain 2-0013P6C4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: FPJ66_RS04825
• Protein ID: WP_003404756.1
• Coordinates: 1023038..1023538 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FPJ66_RS04820
• Protein ID: WP_031653527.1
• Coordinates: 1022565..1023041 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ66_RS04800	1018412..1019683	-	1272	WP_016330387.1	PPE family protein	-
FPJ66_RS04805	1019698..1019997	-	300	WP_003404748.1	PE family protein	-
FPJ66_RS04810	1020441..1022222	+	1782	WP_003404749.1	BCCT family transporter	-
FPJ66_RS04815	1022219..1022476	+	258	WP_003404750.1	hypothetical protein	-
FPJ66_RS04820	1022565..1023041	+	477	WP_031653527.1	DUF1778 domain-containing protein	Antitoxin
FPJ66_RS04825	1023038..1023538	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
FPJ66_RS04835	1023851..1025170	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
FPJ66_RS04840	1025458..1026039	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
FPJ66_RS04845	1026039..1027691	+	1653	WP_003905412.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T132537 WP_003404756.1 NZ_CP041842:1023038-1023538 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T132537 NZ_CP041842:1023038-1023538 [Mycobacterium tuberculosis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17383.99 Da
• Isoelectric Point: 11.0138

>AT132537 WP_031653527.1 NZ_CP041842:1022565-1023041 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRYTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT132537 NZ_CP041842:1022565-1023041 [Mycobacterium tuberculosis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGTACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2