Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3122221..3122791 Replicon chromosome
Accession NZ_CP041841
Organism Mycobacterium tuberculosis strain 2-0021P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ67_RS14675 Protein ID WP_003414166.1
Coordinates 3122221..3122577 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ67_RS14680 Protein ID WP_003901465.1
Coordinates 3122561..3122791 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ67_RS14655 3117673..3119361 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ67_RS14660 3119365..3119691 - 327 WP_003414157.1 hypothetical protein -
FPJ67_RS14665 3119864..3120451 + 588 WP_003914429.1 DUF3558 family protein -
FPJ67_RS14670 3120470..3122119 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ67_RS14675 3122221..3122577 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ67_RS14680 3122561..3122791 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ67_RS14685 3122834..3123877 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ67_RS14690 3124068..3124343 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ67_RS14695 3124519..3124773 - 255 WP_003917684.1 hypothetical protein -
FPJ67_RS14700 3124921..3125325 + 405 WP_009938577.1 hypothetical protein -
FPJ67_RS14705 3125322..3125513 + 192 WP_003414184.1 hypothetical protein -
FPJ67_RS14710 3125577..3126866 + 1290 Protein_2903 transposase family protein -
FPJ67_RS14715 3127100..3127357 + 258 WP_003899489.1 hypothetical protein -
FPJ67_RS14720 3127462..3127773 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T132508 WP_003414166.1 NZ_CP041841:c3122577-3122221 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T132508 NZ_CP041841:c3122577-3122221 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT132508 WP_003901465.1 NZ_CP041841:c3122791-3122561 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT132508 NZ_CP041841:c3122791-3122561 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References