Detailed information of TA system TA132472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 758887..759679
• Replicon: chromosome
• Accession: NZ_CP041841
• Organism: Mycobacterium tuberculosis strain 2-0021P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ67_RS03490
• Protein ID: WP_003403386.1
• Coordinates: 759242..759679 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ67_RS03485
• Protein ID: WP_003403381.1
• Coordinates: 758887..759132 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ67_RS03455	753906..755412	+	1507	Protein_682	carotenoid cleavage oxygenase	-
FPJ67_RS03460	755493..756503	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ67_RS03465	756891..757274	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ67_RS03470	757369..757524	-	156	WP_031582830.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ67_RS03475	757600..758316	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ67_RS03480	758592..758900	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ67_RS03485	758887..759132	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ67_RS03490	759242..759679	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ67_RS03495	759676..759930	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ67_RS03500	760044..762407	+	2364	WP_031582834.1	arylsulfatase AtsD	-
FPJ67_RS03505	762470..762796	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ67_RS03510	762708..763046	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ67_RS03515	763043..763216	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T132472 WP_003403386.1 NZ_CP041841:c759679-759242 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T132472 NZ_CP041841:c759679-759242 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT132472 WP_003403381.1 NZ_CP041841:c759132-758887 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT132472 NZ_CP041841:c759132-758887 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0