Detailed information of TA system TA132446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3082700..3083387
• Replicon: chromosome
• Accession: NZ_CP041840
• Organism: Mycobacterium tuberculosis strain 2-0022P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FPJ68_RS14455
• Protein ID: WP_003414064.1
• Coordinates: 3082992..3083387 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ68_RS14450
• Protein ID: WP_003414061.1
• Coordinates: 3082700..3082966 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ68_RS14425	3078339..3079241	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ68_RS14430	3079310..3080062	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ68_RS14435	3080306..3080581	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ68_RS14440	3080578..3082200	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ68_RS14445	3082287..3082703	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FPJ68_RS14450	3082700..3082966	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ68_RS14455	3082992..3083387	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ68_RS14460	3083384..3083653	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ68_RS14465	3083663..3084757	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ68_RS14470	3084754..3085173	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ68_RS14475	3085247..3085726	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ68_RS14480	3085797..3086597	-	801	WP_003911953.1	thymidylate synthase	-
FPJ68_RS14485	3086753..3087490	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T132446 WP_003414064.1 NZ_CP041840:c3083387-3082992 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T132446 NZ_CP041840:c3083387-3082992 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT132446 WP_003414061.1 NZ_CP041840:c3082966-3082700 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT132446 NZ_CP041840:c3082966-3082700 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64