Detailed information of TA system TA132418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244956..1245524
• Replicon: chromosome
• Accession: NZ_CP041840
• Organism: Mycobacterium tuberculosis strain 2-0022P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ68_RS05875
• Protein ID: WP_003405865.1
• Coordinates: 1245150..1245524 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ68_RS05870
• Protein ID: WP_003405863.1
• Coordinates: 1244956..1245153 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ68_RS05850	1240997..1241635	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ68_RS05855	1241725..1242732	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ68_RS05860	1242749..1243732	-	984	WP_003898731.1	hypothetical protein	-
FPJ68_RS05865	1243795..1244868	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ68_RS05870	1244956..1245153	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ68_RS05875	1245150..1245524	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ68_RS05880	1245727..1246425	+	699	WP_003898733.1	hypothetical protein	-
FPJ68_RS05885	1246543..1246728	+	186	WP_003901093.1	hypothetical protein	-
FPJ68_RS05890	1246655..1246930	-	276	WP_003405867.1	hypothetical protein	-
FPJ68_RS05895	1247173..1247496	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ68_RS05900	1247511..1248221	-	711	WP_003911399.1	hypothetical protein	-
FPJ68_RS05905	1248243..1248452	+	210	WP_003911400.1	hypothetical protein	-
FPJ68_RS05910	1248404..1249111	-	708	Protein_1164	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T132418 WP_003405865.1 NZ_CP041840:1245150-1245524 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T132418 NZ_CP041840:1245150-1245524 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT132418 WP_003405863.1 NZ_CP041840:1244956-1245153 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT132418 NZ_CP041840:1244956-1245153 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07