Detailed information of TA system TA132323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3082216..3082895
• Replicon: chromosome
• Accession: NZ_CP041838
• Organism: Mycobacterium tuberculosis strain 2-0028P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ70_RS14455
• Protein ID: WP_003414059.1
• Coordinates: 3082216..3082632 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ70_RS14460
• Protein ID: WP_003414061.1
• Coordinates: 3082629..3082895 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ70_RS14435	3078268..3079170	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ70_RS14440	3079239..3079991	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ70_RS14445	3080235..3080510	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ70_RS14450	3080507..3082129	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ70_RS14455	3082216..3082632	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ70_RS14460	3082629..3082895	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ70_RS14465	3082921..3083316	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ70_RS14470	3083313..3083582	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ70_RS14475	3083592..3084686	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ70_RS14480	3084683..3085102	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ70_RS14485	3085176..3085655	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ70_RS14490	3085726..3086526	-	801	WP_193051926.1	thymidylate synthase	-
FPJ70_RS14495	3086682..3087419	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T132323 WP_003414059.1 NZ_CP041838:c3082632-3082216 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T132323 NZ_CP041838:c3082632-3082216 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT132323 WP_003414061.1 NZ_CP041838:c3082895-3082629 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT132323 NZ_CP041838:c3082895-3082629 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64