Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2240627..2241213 Replicon chromosome
Accession NZ_CP041837
Organism Mycobacterium tuberculosis strain 2-0029P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FPJ71_RS10380 Protein ID WP_003410010.1
Coordinates 2240627..2240971 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FPJ71_RS10385 Protein ID WP_003410014.1
Coordinates 2240965..2241213 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ71_RS10345 2236333..2236932 + 600 WP_003409989.1 L-lysine exporter -
FPJ71_RS10350 2237348..2237776 + 429 WP_003409992.1 cellulose-binding protein -
FPJ71_RS10355 2238002..2238541 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FPJ71_RS10360 2239061..2239621 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FPJ71_RS10365 2239618..2239959 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FPJ71_RS10370 2240045..2240302 + 258 WP_003410006.1 hypothetical protein -
FPJ71_RS10375 2240203..2240538 - 336 WP_003410009.1 dehydrogenase -
FPJ71_RS10380 2240627..2240971 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FPJ71_RS10385 2240965..2241213 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FPJ71_RS10390 2241313..2243628 - 2316 WP_031582628.1 cation transporter ATPase CptG -
FPJ71_RS10395 2243625..2243897 - 273 WP_003410017.1 DUF1490 family protein -
FPJ71_RS10400 2243950..2244306 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FPJ71_RS10405 2244463..2245230 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T132249 WP_003410010.1 NZ_CP041837:c2240971-2240627 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T132249 NZ_CP041837:c2240971-2240627 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT132249 WP_003410014.1 NZ_CP041837:c2241213-2240965 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT132249 NZ_CP041837:c2241213-2240965 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References