Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1773307..1773920 Replicon chromosome
Accession NZ_CP041837
Organism Mycobacterium tuberculosis strain 2-0029P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag FPJ71_RS08225 Protein ID WP_003407786.1
Coordinates 1773516..1773920 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag FPJ71_RS08220 Protein ID WP_009935474.1
Coordinates 1773307..1773510 (+) Length 68 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ71_RS08190 1768712..1769092 + 381 WP_003407768.1 fumarate reductase subunit C -
FPJ71_RS08195 1769089..1769466 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
FPJ71_RS08200 1769534..1770142 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
FPJ71_RS08205 1770326..1771474 + 1149 Protein_1618 MMPL family transporter -
FPJ71_RS08210 1771484..1771930 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
FPJ71_RS08215 1771965..1773254 + 1290 WP_003407781.1 threonine ammonia-lyase -
FPJ71_RS08220 1773307..1773510 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ71_RS08225 1773516..1773920 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
FPJ71_RS08230 1773937..1775679 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
FPJ71_RS08235 1775672..1777969 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-124)

Antitoxin

(2-44)


Sequences


Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T132241 WP_003407786.1 NZ_CP041837:1773516-1773920 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T132241 NZ_CP041837:1773516-1773920 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT132241 WP_009935474.1 NZ_CP041837:1773307-1773510 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT132241 NZ_CP041837:1773307-1773510 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6A7V


Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References